Expression and fine structure of the gene encoding N epsilon-(indole-3-acetyl)-L-lysine synthetase from Pseudomonas savastanoi.
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چکیده
منابع مشابه
Microbial synthesis and degradation of indole-3-acetic acid. I. The conversion of L-tryptophan to indole-3-acetamide by an enzyme system from Pseudomonas savastanoi.
An enzyme which catalyzes the conversion of L-tryptophan to indole-3-acetamide has been isolated from cells of Pseudomonas savastanoi and purified 89-fold. The enzyme catalyzes a reaction in which 1 mole of L-tryptophan and 1 mole of 02 are consumed and 1 mole of indole-3-acetamide and 1 mole of CO2 are produced. The enzyme has a pH optimum between 7.2 and 9.2. It is specific for the L isomer o...
متن کاملNew insights into the role of indole-3-acetic acid in the virulence of Pseudomonas savastanoi pv. savastanoi.
Indole-3-acetic acid (IAA) is a widespread phytohormone among plant-associated bacteria, including the tumour-inducing pathogen of woody hosts, Pseudomonas savastanoi pv. savastanoi. A phylogenetic analysis of the iaaM/iaaH operon, which is involved in the biosynthesis of IAA, showed that one of the two operons encoded by Pseudomonas savastanoi pv. savastanoi NCPPB 3335, iaaM-1/iaaH-1, is horiz...
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15 صفحه اولStructure-activity relationship in the urokinase hydrolysis of alpha-N-acetyl-L-lysine anilides.
The absence of both nonproductive binding and substrate activation and also the good solubility of the substrates make the urokinase-catalysed hydrolysis of specific anilides a very suitable reaction for substrate structure-enzyme activity studies. Derivatives of alpha-N-acetyl-L-lysine anilide with high sigma minus-value substituents in the aniline ring were synthesized. Rate constants kappa-c...
متن کاملMolecular characterization and expression analysis of the anthranilate synthase gene of Pseudomonas syringae subsp. savastanoi.
The trpE gene, which encodes the large component of the enzyme anthranilate synthase, was isolated from a Pseudomonas syringae subsp. savastanoi (P. savastanoi) cosmid library. Cosmids that complemented an Escherichia coli trpE mutation contained a gene whose product is 86% homologous at the deduced amino acid level to TrpE of Pseudomonas aeruginosa and Pseudomonas putida. Amino acid sequence c...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1990
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.87.15.5797